Abstract
A cross-polarization scheme is presented to filter out the excitation light from the emission spectrum of fluorescent dyes using green light emitting diodes as a light source and a linear charge coupled device as an intensity detector. The excitation light was linearly polarized and was then used to illuminate the fluorescent dyes in the microchannels of a capillary electrophoresis microchip. The detector was shielded by the second polarizer, oriented perpendicular to the excitation light. The fluorescent signals from Rhodamine B dyes were measured in a dilution series with resulting emission signals and four different concentrations of fluorescent dyes were detected simultaneously with the same excitation source and detector. A limit-of-detection of 1 μM was demonstrated for Rhodamine B dye under the optimal conditions.
©2012 Optical Society of America
Full Article | PDF ArticleMore Like This
Oluwatosin O. Dada
Appl. Opt. 59(16) 4849-4855 (2020)
Chaitanya Dongre, Ronald Dekker, Hugo J. W. M. Hoekstra, Markus Pollnau, Rebeca Martinez-Vazquez, Roberto Osellame, Giulio Cerullo, Roberta Ramponi, Rob van Weeghel, Geert A. J. Besselink, and Hans H. van den Vlekkert
Opt. Lett. 33(21) 2503-2505 (2008)
Benjamin R. Watts, Zhiyi Zhang, Chang-Qing Xu, Xudong Cao, and Min Lin
Biomed. Opt. Express 3(11) 2784-2793 (2012)