Table 1.
Fluorescence Bands Selected for Excitation Sources at 405 and 470 nm
Disease | Excitation 405 nm | Excitation 470 nm |
---|
Citrus canker and citrus scab (Group 1) | Filters at 550, 580, and 690 nm | Filters at 550, 570, and 690 nm |
HLB and zinc deficiency (Group 2) | Filters at 560, 580, and 690 nm | Filters at 530, 550, and 690 nm |
Table 2.
Classification Results for Group 1 using True Positive (TP), True Negative (TN), False Negative (FN), and False Positive (FP) Samples
| | | Condition Determined by Assay Value |
---|
| | | Condition Positive | Condition Negative |
---|
Excitation 405 nm | F550 | Test outcome positive | | |
Test outcome negative | | |
F580 | Test outcome positive | | |
Test outcome negative | | |
F690 | Test outcome positive | | |
Test outcome negative | | |
Excitation 470 nm | F550 | Test outcome positive | | |
Test outcome negative | | |
F570 | Test outcome positive | | |
Test outcome negative | | |
F690 | Test outcome positive | | |
Test outcome negative | | |
Table 3.
Sensitivity, Specificity, and Accuracy Parameters for Citrus Canker Detection (Group 1)
| Excitation 405 nm | Excitation 470 nm |
---|
| F550 | F580 | F690 | F550 | F570 | F690 |
---|
Sensitivity | 100% | 100% | 80% | 95.6% | 100% | 67.8% |
Specificity | 62.2% | 61.1% | 25.6% | 54.4% | 12.2% | 87.8% |
Accuracy | 81.1% | 80.6% | 52.8% | 75% | 56.1% | 77.8% |
Table 4.
Classification Results for Group 2 using True Positive (TP), True Negative (TN), False Negative (FN) and False Positive (FP) Samples
| | | Condition Determined by Assay Value |
---|
| | | Condition Positive | Condition Negative |
---|
Excitation 405 nm | F560 | Test outcome positive | | |
Test outcome negative | | |
F580 | Test outcome positive | | |
Test outcome negative | | |
F690 | Test outcome positive | | |
Test outcome negative | | |
Excitation 470 nm | F530 | Test outcome positive | | |
Test outcome negative | | |
F550 | Test outcome positive | | |
Test outcome negative | | |
F690 | Test outcome positive | | |
Test outcome negative | | |
Table 5.
Sensitivity, Specificity, and Accuracy Parameters for HLB Detection (Group 2)
| Excitation 405 nm | Excitation 470 nm |
---|
| F560 | F580 | F690 | F530 | F550 | F690 |
---|
Sensitivity | 100% | 0% | 7.4% | 90.1% | 1.2% | 56.8% |
Specificity | 6.1% | 100% | 100% | 23.2% | 100% | 83.8% |
Accuracy | 48.3% | 55% | 58.3% | 53.3% | 55.6% | 71.7% |
Table 6.
Sensitivity, Specificity, and Accuracy Parameters Considering Combination for Citrus Canker Detection (Group 1)
| Excitation 405 nm | Excitation 470 nm | Combination of the Two Excitations |
---|
| F550 + F580 | F550 + F580 + F690 | F550 + F690 | F550 + F570 + F690 | All Fluorescence |
---|
Sensitivity | 100% | 100% | 96.7% | 100% | 100% |
Specificity | 68.9% | 76.7% | 93.3% | 93.3% | 95.6% |
Accuracy | 84.4% | 88.3% | 95% | 96.7% | 97.8% |
Table 7.
Sensitivity, Specificity, and Accuracy Parameters Considering the Combinations for HLB Detection (Group 2)
| Excitation 405 nm | Excitation 470 nm | Combination of the Two Excitations |
---|
| F580 + F690 | F560 + F580 + F690 | F550 + F690 | F530 + F550 + F690 | All Fluorescence |
---|
Sensitivity | 7.4% | 100% | 59.7% | 96.3% | 100% |
Specificity | 100% | 90.9% | 100% | 90.9% | 90.9% |
Accuracy | 58.3% | 95% | 80.6% | 93.3% | 95% |