Abstract
We studied the fluorescence properties of several potential picosecond lifetime standards suitable for two-photon excitation from a Ti: sapphire femtosecond laser. The fluorescence emission of the selected fluorophores (rose bengal, pyridine 1, and LDS 798) covered the visible to near-infrared wavelength range from 550 to 850 nm. We suggest that these compounds can be used to measure the appropriate instrument response functions needed for accurate deconvolution of fluorescence lifetime data. Lifetime measurements with multiphoton excitation that use scatterers as a reference may fail to properly resolve fluorescence intensity decays. This is because of the different sensitivities of photodetectors in different spectral regions. Also, detectors often lose sensitivity in the near-infrared region. We demonstrate that the proposed references allow a proper reconvolution of measured lifetimes. We believe that picosecond lifetime standards for two-photon excitation will find broad applications in multiphoton spectroscopy and in fluorescence lifetime imaging microscopy (FLIM).
PDF Article
More Like This
Application of ultrafast gold luminescence to measuring the instrument response function for multispectral multiphoton fluorescence lifetime imaging
Clifford B. Talbot, Rakesh Patalay, Ian Munro, Sean Warren, Fulvio Ratto, Paolo Matteini, Roberto Pini, H. Georg Breunig, Karsten König, Antony C. Chu, Gordon W. Stamp, Mark A. A. Neil, Paul M. W. French, and Chris Dunsby
Opt. Express 19(15) 13848-13861 (2011)
Cited By
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription