Abstract
We describe a technique to enhance both the weak-signal relative sensitivity and the dynamic range of a laser scanning optical microscope. The technique is based on maintaining a fixed detection power by fast feedback control of the illumination power, thereby transferring high measurement resolution to weak signals while virtually eliminating the possibility of image saturation. We analyze and demonstrate the benefits of adaptive illumination in two-photon fluorescence microscopy.
© 2007 Optical Society of America
Full Article | PDF ArticleMore Like This
Kengyeh K. Chu, Daryl Lim, and Jerome Mertz
Biomed. Opt. Express 1(1) 236-245 (2010)
A. J. Wright, S. P. Poland, J. M. Girkin, C. W. Freudiger, C. L. Evans, and X. S. Xie
Opt. Express 15(26) 18209-18219 (2007)
Cathie Ventalon, Rainer Heintzmann, and Jerome Mertz
Opt. Lett. 32(11) 1417-1419 (2007)