Abstract
We propose and demonstrate a novel differential two-signal technique of coherent anti-Stokes Raman scattering (CARS) imaging microscopy using a picosecond (ps) optical parametric oscillator (OPO). By adjusting a Lyot filter inside the cavity, we operated the OPO oscillating in two stable modes separated by a few nanometers. The CARS images generated by the two modes are separated by a spectrograph behind the microscope setup, and their differential image is directly obtained by balanced lock-in detection. The feasibility of the technique is experimentally verified by imaging micrometer-sized polystyrene beads immersed in water.
© 2007 Optical Society of America
Full Article | PDF ArticleMore Like This
Chao-Yu Chung, Yen-Yin Lin, Kuo-Yu Wu, Wan-Yu Tai, Shi-Wei Chu, Yao-Chang Lee, Yeukuang Hwu, and Yin-Yu Lee
Opt. Express 18(6) 6116-6122 (2010)
Israel Rocha-Mendoza, Wolfgang Langbein, Peter Watson, and Paola Borri
Opt. Lett. 34(15) 2258-2260 (2009)
Haifeng Wang, Terry B. Huff, Yan Fu, Kevin Y. Jia, and Ji-Xin Cheng
Opt. Lett. 32(15) 2212-2214 (2007)