Abstract
We demonstrate a form of scanning microscopy using a short-focal-length Fresnel zone plate and a low-NA relay telescope. Owing to a focal length of only , the zone plate produces large wavefront tilt and consequently severe vignetting for off-axis illumination. By scanning an optically trapped fluorescent sphere, we measure the vignetted collection region of the zone-plate imaging system. The fluorescence collection efficiency is sharply peaked and has a lateral width of , which agrees with numerical simulations.
© 2009 Optical Society of America
Full Article | PDF ArticleMore Like This
Olov von Hofsten, Michael Bertilson, Julia Reinspach, Anders Holmberg, Hans M. Hertz, and Ulrich Vogt
Opt. Lett. 34(17) 2631-2633 (2009)
Germain Boivin
Appl. Opt. 16(4) 1070-1073 (1977)
Di Feng
J. Opt. Soc. Am. A 31(9) 2070-2074 (2014)