Abstract
We present the first demonstration, to our knowledge, of a label-free flow cytometer for the analysis of biological specimens using multiplex coherent anti-Stokes Raman scattering (MCARS) and elastic scatter measurements. The MCARS system probes the Raman vibrational energy levels and the elastic scatter provides morphological information. We demonstrate these capabilities by probing a culture of Saccharomyces cerevisiae at and constructing a background-free Raman reconstruction using a Kramers–Kronig relation. A theoretical analysis shows that this system could operate at speeds of with appropriate hardware; thus facilitating integration into commercial flow cytometers or use as a high-speed, stand-alone device.
© 2011 Optical Society of America
Full Article | PDF ArticleMore Like This
Charles H. Camp Jr., Siva Yegnanarayanan, Ali A. Eftekhar, Hamsa Sridhar, and Ali Adibi
Opt. Express 17(25) 22879-22889 (2009)
Chi Zhang, Kai-Chih Huang, Bartek Rajwa, Junjie Li, Shiqi Yang, Haonan Lin, Chien-sheng Liao, Gregory Eakins, Shihuan Kuang, Valery Patsekin, J. Paul Robinson, and Ji-Xin Cheng
Optica 4(1) 103-109 (2017)
K. O’Dwyer, R. Mouras, A. A. Mani, D. Rice, M. Gleeson, N. Liu, S. A. M. Tofail, and C. Silien
Appl. Opt. 57(22) E32-E36 (2018)