Abstract
We describe the corkscrew point spread function (PSF), which can localize objects in three dimensions throughout a depth of field with nanometer precision. The corkscrew PSF rotates as a function of the axial (z) position of an emitter. Fisher information calculations show that the corkscrew PSF can achieve nanometer localization precision with limited numbers of photons. We demonstrate three-dimensional super-resolution microscopy with the corkscrew PSF by imaging beads on the surface of a triangular polydimethylsiloxane (PDMS) grating. With 99,000 photons detected, the corkscrew PSF achieves a localization precision of in x, in y, and in z.
© 2011 Optical Society of America
Full Article | PDF ArticleMore Like This
James M. Jusuf and Matthew D. Lew
Opt. Express 30(20) 37154-37174 (2022)
Abhijit Marar and Peter Kner
Opt. Lett. 45(2) 591-594 (2020)
Bin Yu, Jie Yu, Weihai Li, Bo Cao, Heng Li, Danni Chen, and Hanben Niu
Appl. Opt. 55(3) 449-453 (2016)