Abstract
This Letter presents an enhanced temporal focusing-based multiphoton excitation (MPE) microscope in which the conventional diffraction grating is replaced by a digital micromirror device (DMD). Experimental results from imaging a thin fluorescence film show that the 4.0 μm axial resolution of the microscope is comparable with that of a setup incorporating a grating; hence, the optical sectioning ability of the proposed setup is demonstrated. Similar to a grating, the DMD diffracts illuminating light frequencies for temporal focusing; additionally, it generates arbitrary patterns. Since the DMD is placed on the image–conjugate plane of the objective lens’ focal plane, the MPE pattern can be projected on the focal plane precisely.
© 2014 Optical Society of America
Full Article | PDF ArticleMore Like This
Li-Chung Cheng, Chi-Hsiang Lien, Yong Da Sie, Yvonne Yuling Hu, Chun-Yu Lin, Fan-Ching Chien, Chris Xu, Chen Yuan Dong, and Shean-Jen Chen
Biomed. Opt. Express 5(8) 2526-2536 (2014)
Chia-Yuan Chang, Yvonne Yuling Hu, Chun-Yu Lin, Cheng-Han Lin, Hsin-Yu Chang, Sheng-Feng Tsai, Tzu-Wei Lin, and Shean-Jen Chen
Biomed. Opt. Express 7(5) 1727-1736 (2016)
Chi-Hsiang Lien, Chun-Yu Lin, Shean-Jen Chen, and Fan-Ching Chien
Opt. Express 22(22) 27290-27299 (2014)