Abstract
We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber, and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral purity. Selective excitation at different wavelengths permits simultaneous imaging of two fluorescent markers at a nanoscale resolution in three dimensions.
© 2015 Optical Society of America
Full Article | PDF ArticleMore Like This
Dominik Wildanger, Eva Rittweger, Lars Kastrup, and Stefan W. Hell
Opt. Express 16(13) 9614-9621 (2008)
Jiaxin Yu, Xianhe Sun, Fuhong Cai, Zhenfeng Zhu, Anjun Qin, Jun Qian, Benzhong Tang, and Sailing He
Opt. Lett. 40(10) 2313-2316 (2015)
Christian Osseforth, Jeffrey R. Moffitt, Lothar Schermelleh, and Jens Michaelis
Opt. Express 22(6) 7028-7039 (2014)