Restoring Single-Molecule Localizations with Wavefront Sensing Adaptive Optics for Deep-Tissue Super-Resolution Imaging

Sanghyeon Park; Sanghyeon Park; Yonghyeon Jo; Yonghyeon Jo; Minsu Kang; Minsu Kang; Jin Hee Hong; Sang-Hee Shim; Sang-Hee Shim; Wonshik Choi; Wonshik Choi

doi:10.1364/AOA.2022.OTh5B.4

Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP)
Technical Digest Series (Optica Publishing Group, 2022),
paper OTh5B.4
•https://doi.org/10.1364/AOA.2022.OTh5B.4

Restoring Single-Molecule Localizations with Wavefront Sensing Adaptive Optics for Deep-Tissue Super-Resolution Imaging

Sanghyeon Park, Yonghyeon Jo, Minsu Kang, Jin Hee Hong, Sang-Hee Shim, and Wonshik Choi

Adaptive Optics and Applications 2022

Vancouver, British Columbia Canada
11–15 July 2022
ISBN: 978-1-957171-09-8

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Microscopy III (OTh5B)

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S. Park, Y. Jo, M. Kang, J. H. Hong, S. Shim, and W. Choi, "Restoring Single-Molecule Localizations with Wavefront Sensing Adaptive Optics for Deep-Tissue Super-Resolution Imaging," in Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP), Technical Digest Series (Optica Publishing Group, 2022), paper OTh5B.4.

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Abstract

We measured the sample-induced aberration using reflectance signal without resorting to fluorescence detection and corrected it. It enabled deep-tissue single-molecule localization microscopy even when the aberration was too severe to initiate localization.

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