Measurement of transient heating in cells during free-electron laser microirradiation

Curtis F. Chapman; Bruce J. Tromberg; Michael W. Berns; Kris A. Peterson; Kris A. Peterson; Andrew G. Jeung; Andrew G. Jeung; H. Alan Schwettman; H. Alan Schwettman

The Pacific Rim Conference on Lasers and Electro-Optics
Technical Digest Series (Optica Publishing Group, 1995),
paper ThF3

Measurement of transient heating in cells during free-electron laser microirradiation

Curtis F. Chapman, Bruce J. Tromberg, Michael W. Berns, Kris A. Peterson, Andrew G. Jeung, and H. Alan Schwettman

Conference on Lasers and Electro-Optics/Pacific Rim 1995

Chiba Japan
10–14 July 1995
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Medical and Biological Applications I (ThF)

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C. F. Chapman, B. J. Tromberg, M. W. Berns, K. A. Peterson, A. G. Jeung, and H. A. Schwettman, "Measurement of transient heating in cells during free-electron laser microirradiation," in The Pacific Rim Conference on Lasers and Electro-Optics, Technical Digest Series (Optica Publishing Group, 1995), paper ThF3.

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Abstract

Infrared absorption data can yield useful information about the molecular structure of cells. Since IR spectra are difficult to obtain from single cells, we have developed a technique called fluorescence detected infrared (FDIR) spectroscopy (Tromberg, et al., 1993), which uses the relatively high quantum efficiency of fluorescence to indicate IR absorption and a free electron laser (FEL) to provide the wavelength-tunable IR energy.

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