Abstract

The fluorescence enhancement is induced by Grating-coupled surface plasmon resonance (GC-SPR) of a plasmonic chip in which a wavelength-sized periodic structure is coated with a thin metal film. In the fluorescence microscopic images of nanoparticles adsorbed on the plasmonic chip observed with transmitted light, the bright spot based on the nanoantenna effect was observed at the center of a Bull's eye pattern composed of concentric circles in a plasmonic chip. In this study, resonance wavelength was controlled by SiO₂ film thickness deposited on a thin Ag film and the excitation and emission enhancement was individually evaluated by an upright-inverted microscope to clarify the cause of the nanoantenna effect. As the observation result, nanoantenna effect was larger in SiO₂ 80 nm than SiO₂ 20 nm.

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