Abstract

Steady-state and time-resolved fluorescence have been approached as two different methodologies in fluorescence studies. The fact is that both have been applied successfully to study the physical properties and as a fingerprint to differentiate among various physiological states.^{1 3} We wanted to combine both techniques in one design and have the advantage of simultaneously acquiring both static and dynamic fluorescence characteristics of the compounds under examination. We introduce a method for the acquisition of both spectral and temporal fluorescence emission from tissue chromophores after monochromatic excitation.

© 1994 IEEE