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Frequency content of the double-helix PSF for 3D microscopy in the presence of spherical aberration

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We examine the Fourier content of the double helix point-spread function (DH-PSF) by computing the DH optical transfer function (OTF). DH-OTFs are compared to OTFs of conventional fluorescence microscopy in the presence of spherical aberration.

© 2011 Optical Society of America

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