Fluence rate effects in meta-tetra(hydroxyphenyl)chlorin (mTHPC) photosensitized HT29 multiceli tumor spheroids Comparative study with Colo26 spheroids

Estelle Maugain; Stéphanie Coutier; Lina N. Bezdetnaya; Sophie Marchai; Jean-Louis Merlin; Jacques Didelon; François Guillemin; François Guillemin

doi:10.1364/ECBO.2001.4433_148

Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy
Technical Digest Series (Optica Publishing Group, 2001),
paper 4433_148
•https://doi.org/10.1364/ECBO.2001.4433_148

Fluence rate effects in meta-tetra(hydroxyphenyl)chlorin (mTHPC) photosensitized HT29 multiceli tumor spheroids Comparative study with Colo26 spheroids

Estelle Maugain, Stéphanie Coutier, Lina N. Bezdetnaya, Sophie Marchai, Jean-Louis Merlin, Jacques Didelon, and François Guillemin

European Conference on Biomedical Optics 2001

Munich Germany
18–21 June 2001
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Photodynamic Therapy III (4433)

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E. Maugain, S. Coutier, L. N. Bezdetnaya, S. Marchai, J. Merlin, J. Didelon, and F. Guillemin, "Fluence rate effects in meta-tetra(hydroxyphenyl)chlorin (mTHPC) photosensitized HT29 multiceli tumor spheroids Comparative study with Colo26 spheroids," in Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy, Technical Digest Series (Optica Publishing Group, 2001), paper 4433_148.

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Abstract

The present study evaluates the impact of the fluence rate of red light irradiation (λ = 650 nm) on photodynamic efficacy in mTHPC-sensitized HT29 and Colo26 spheroids. Photocytotoxicity, computed as product of the cell yield and plating efficiency, increased progressively when the fluence rate was reduced from 90 to 30 mW cm^-2 in both types of spheroids. The observed photocytotoxicity was mainly due to an immediate rather than a delayed cell death for HT29 spheroids. An opposite effect was observed for Colo26 spheroids. The plausible explanations could be the difference in penetration of mTHPC into the spheroids as well as their heterogeneous structure.

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