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  • Advanced Microscopy Techniques III
  • SPIE Proceedings (Optica Publishing Group, 2013),
  • paper 879703

Techniques for 3D tracking of single molecules with nanometer accuracy in living cells

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Abstract

We describe a microscopy technique that, combining wide-field single molecule microscopy, bifocal imaging and Highly Inclined and Laminated Optical sheet (HILO) microscopy, allows a 3D tracking with nanometer accuracy of single fluorescent molecules in vitro and in living cells.

© 2013 SPIE

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