Detecting DNA-Ligand Electrostatic Interactions With a FRET-Based Probe

Chandrashekhar U. Murade; George T. Shubeita

doi:10.1364/3D.2022.JW5D.5

Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP)
Technical Digest Series (Optica Publishing Group, 2022),
paper JW5D.5
•https://doi.org/10.1364/3D.2022.JW5D.5

Detecting DNA-Ligand Electrostatic Interactions With a FRET-Based Probe

Chandrashekhar U. Murade and George T. Shubeita

Imaging Systems and Applications 2022

Vancouver, British Columbia Canada
11–15 July 2022
ISBN: 978-1-957171-09-8

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Joint Postdeadline Paper Session IV (JW5D)

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C. U. Murade and G. T. Shubeita, "Detecting DNA-Ligand Electrostatic Interactions With a FRET-Based Probe," in Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP), Technical Digest Series (Optica Publishing Group, 2022), paper JW5D.5.

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Abstract

DNA-ligand interactions are dominated by electrostatics as DNA is a highly charged molecule at physiological conditions. Here, we present a FRET-based sensor which can optically report on these interactions between DNA and charged ligands.

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