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Time-resolved 3D Tracking of Individual Quantum Dot Labeled Proteins in Live Cells via Confocal Feedback

James H. Werner, M. Lisa Phipps, Peter M. Goodwin, Patrick J. Cutler, Diane S. Lidke, and Bridget S. Wilson

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Abstract

We have developed a microscope system that uses active feed-back to follow individual quantum dot labeled proteins moving in three dimensions in live cells at μm/s transport velocities with 100 picoseconds temporal resolution.

© 2010 Optical Society of America

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