Detection of Rare Circulating Tumor Cell Clusters in Whole Blood Using Label-free, Flow Cytometry

Nilay Vora; Prashant Shekhar; Michael Esmail; Abani Patra; Irene Georgakoudi

doi:10.1364/MICROSCOPY.2022.MW3A.3

Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN)
Technical Digest Series (Optica Publishing Group, 2022),
paper MW3A.3
•https://doi.org/10.1364/MICROSCOPY.2022.MW3A.3

Detection of Rare Circulating Tumor Cell Clusters in Whole Blood Using Label-free, Flow Cytometry

Nilay Vora, Prashant Shekhar, Michael Esmail, Abani Patra, and Irene Georgakoudi

Microscopy Histopathology and Analytics 2022

Fort Lauderdale, Florida United States
24–27 April 2022
ISBN: 978-1-957171-03-6

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Translational IV (MW3A)

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N. Vora, P. Shekhar, M. Esmail, A. Patra, and I. Georgakoudi, "Detection of Rare Circulating Tumor Cell Clusters in Whole Blood Using Label-free, Flow Cytometry," in Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN), Technical Digest Series (Optica Publishing Group, 2022), paper MW3A.3.

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Abstract

We demonstrate the potential of in vitro, label-free detection of Circulating Tumor Cell Clusters in whole blood using confocal backscatter and florescence flow cytometry along with a machine learning drive peak detection/classification algorithm.

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