Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Quantitative live cell imaging with tomographic phase microscopy

Not Accessible

Your library or personal account may give you access


In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

© 2009 Optical Society of America

PDF Article
More Like This
Quantitative Phase imaging of Live Yeast cells using Digital Holographic Microscopy

P. Vimal Prabhu and Renu John
S5A.4 International Conference on Fibre Optics and Photonics (Photonics) 2014

3-D Imaging of Malaria-infected Human Red Blood Cells Using Optical Diffraction Tomography

Kyoohyun Kim, HyeOk Yoon, and YongKeun Park
SM4P.2 CLEO: Science and Innovations (CLEO_SI) 2014

Tomographic phase microscopy for label-free imaging in biomedicine

P. Ferraro, F. Merola, P. Memmolo, L. Miccio, M. Mugnano, M. Villone, and P.L. Maffettone
JW3A.116 Frontiers in Optics (FiO) 2018


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access Optica Member Subscription

Select as filters

Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved