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Optica Publishing Group
  • Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)
  • OSA Technical Digest (Optica Publishing Group, 2019),
  • paper NS2B.3
  • https://doi.org/10.1364/NTM.2019.NS2B.3

Single-shot 3D fluorescence microscopy with Fourier DiffuserCam

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Abstract

We propose a single-shot 3D fluorescence microscope that achieves large field-of-view and good resolution across a wide axial range. This is implemented by inserting a diffuser into the Fourier plane of the objective and solving an inverse problem. We show experimental 3D results of a freely-moving C. elegans nematode at 25 fps.

© 2019 The Author(s)

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