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Conferences > ODF > 2022 > OThP4B > OThP4B_03
  • Proceedings of the 2022 International Symposium on Imaging, Sensing, and Optical Memory (ISOM) and the 13th International Conference on Optics-photonics Design and Fabrication (ODF)
  • Technical Digest Series (Optica Publishing Group, 2022),
  • paper OThP4B_03

Experimental Verification of Fluorescence Tags Using FRET Networks Responding to Molecular Inputs

Keita Hayashi, Yusuke Ogura, Suguru Shimomura, Takahiro Nishimura, and Jun Tanida

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Abstract

Multiplexed fluorescence sensing/imaging is an important technique for elucidating cellular activity by simultaneously visualizing the behavior of biomolecules. In this paper, we present experimental verification of a fluorescence tag whose fluorescence spectrum can be switched with the change of the FRET network’s structure in response to molecular inputs. An unmixing algorithm is applied to estimate the concentration of a target molecule. Experimental results demonstrate the basic function of the fluorescence tag for quantitative molecular sensing.

© 2022 IEEE

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