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Mapping intracellular temperature using Green Fluorescent Protein – From in vitro to in vivo

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Abstract

We report the use of green fluorescent protein (GFP) as a thermal nanoprobe suited for intracellular temperature mapping. Temperature probing is achieved by monitoring the fluorescence polarization anisotropy of GFP. The method is tested on GFP-transfected cancer cell lines enabling a spatial resolution of 300 nm and a temperature accuracy of 0.4°C [1]. The technique has recently been extended to measure intracellular temperature in an in vivo model of transfected C Elegans.

© 2013 Optical Society of America

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