Fluorescence lifetime and structural heterogeneity of fluorescent pH biosensors

Franz-Josef Schmitt; Judith Reisdorf; Amna Shah Mehmood; Farzin Ghane Golmohamadi; Jan Laufer

doi:10.1364/OTS.2022.OS3D.5

Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN)
Technical Digest Series (Optica Publishing Group, 2022),
paper OS3D.5
•https://doi.org/10.1364/OTS.2022.OS3D.5

Fluorescence lifetime and structural heterogeneity of fluorescent pH biosensors

Franz-Josef Schmitt, Judith Reisdorf, Amna Shah Mehmood, Farzin Ghane Golmohamadi, and Jan Laufer

Optical Tomography and Spectroscopy 2022

Fort Lauderdale, Florida United States
24–27 April 2022
ISBN: 978-1-957171-03-6

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Photonics and Ultrasound I (OS3D)

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F. Schmitt, J. Reisdorf, A. S. Mehmood, F. G. Golmohamadi, and J. Laufer, "Fluorescence lifetime and structural heterogeneity of fluorescent pH biosensors," in Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN), Technical Digest Series (Optica Publishing Group, 2022), paper OS3D.5.

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Abstract

Fluorescent proteins with flexible structures show lifetime heterogeneity and stronger pH sensitivity compared to rigid variants. Molecules with static conformations exhibit distinct lifetime components for protonated and deprotonated forms.

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