Evaluation of Barcodes Reveals Ligation Biases between the Terminal Bases

Jing Tu; Na Lu; Qinyu Ge; Zuhong Lu

doi:10.1364/PIBM.2017.T2C.3

International Conference on Photonics and Imaging in Biology and Medicine
(Optica Publishing Group, 2017),
paper T2C.3
•https://doi.org/10.1364/PIBM.2017.T2C.3

Evaluation of Barcodes Reveals Ligation Biases between the Terminal Bases

Jing Tu, Na Lu, Qinyu Ge, and Zuhong Lu

International Conference on Photonics and Imaging in Biology and Medicine 2017

Suzhou China
26–28 September 2017
ISBN: 978-1-943580-35-4

From the session
Annual Symposium for KLBMP and SKLB II (T2C)

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J. Tu, N. Lu, Q. Ge, and Z. Lu, "Evaluation of Barcodes Reveals Ligation Biases between the Terminal Bases," in International Conference on Photonics and Imaging in Biology and Medicine, (Optica Publishing Group, 2017), paper T2C.3.

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Abstract

Barcode-independent biases accompany with the application of barcodes in high throughput sequencing. We evaluate two types of barcodes on a sequencing-by-ligation platform. The results suggest that terminal bases of barcodes tend to influence ligation.

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