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Sub-Picosecond Resonance Raman Spectroscopy of Biological Systems

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Abstract

We have studied the photochemical and photophysical events that occur upon excitation of two important biological molecules, bacteriorhodopsin (bR) and ligated hemoglobin (Hb) using a sub-picosecond resonance Raman flow technique. The laser is a Spectra Physics YAG laser which is compressed and frequency-doubled and which synchronously pumps a cavity-dumped dye laser. In this one-color technique, the transients observed are those appearing within the laser pulse of 700-800 fs.

© 1990 Optical Society of America

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