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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 45,
  • Issue 2,
  • pp. 272-278
  • (1991)

Quantitative Treatment of UV Resonance Raman Spectra of Biological Molecules: Application to the Study of Membrane-Bound Proteins

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Abstract

A set of instrumental and digital signal processing techniques (Fourier self-deconvolution and the maximum entropy method) was adopted to detect and treat low-signal-level UV resonance Raman spectra of biological molecules and supramolecular complexes. The applicability of the methods for analysis of ATP binding in the active site of Na<sup>+</sup>,K<sup>+</sup>-ATPase, as well as for investigation of aromatic amino acids involved in the functioning of bacterial and visual rhodopsins, is demonstrated.

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