Abstract
In this work, a microwave assisted method was developed for
synthesis of red fluorescent copper nanoclusters (NCs) using trypsin as a
template (trypsin–Cu). The as-synthesized trypsin–Cu NCs are
stable and water soluble, exhibiting fluorescence emission at 657 nm when
excited at 490 nm. The as-prepared red-emitting trypsin–Cu NCs were
characterized by using several analytical techniques such as
ultraviolet–visible (UV–Vis) and fluorescence, fluorescence
lifetime, Fourier transform infrared, and X-ray photoelectron
spectroscopic techniques. Red-emitting trypsin–Cu NCs acted as a
nanosensor for sensing both Pb2+ and
Hg2+ ions through fluorescence quenching. Using this
approach, good linearities are observed in the range of 0.1–25 and
of 0.001–1 μM with the lower limit of detection of 14.63 and
56.81 nM for Pb2+ and Hg2+ ions,
respectively. Trypsin–Cu NCs-based fluorescence assay was
successfully applied to detect both Hg2+ and
Pb2+ ions in water and tobacco samples.
© 2022 The Author(s)
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