Abstract

Structured illumination microscopy (SIM) is an established technique that allows subdiffraction resolution imaging by heterodyning high sample frequencies into the system’s passband via structured illumination. However, until now, SIM has been typically used to achieve subdiffraction resolution for intensity-based imaging. Here, we present a novel optical setup that uses structured illumination with a broadband light source to obtain noise-reduced, subdiffraction resolution, quantitative phase imaging (QPM) of cells. We compare this with a previous work for subdiffraction QPM imaging via SIM that used a laser source, and was thus still corrupted by coherent noise.

© 2014 Optical Society of America

Structured illumination quantitative phase microscopy for enhanced resolution amplitude and phase imaging

Shwetadwip Chowdhury and Joseph Izatt
Biomed. Opt. Express 4(10) 1795-1805 (2013)

Structured illumination contrast transfer function for high resolution quantitative phase imaging

Sibi Chakravarthy Shanmugavel and Yunhui Zhu
Opt. Express 31(24) 40151-40165 (2023)

Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation

Tomáš Lukeš, Pavel Křížek, Zdeněk Švindrych, Jakub Benda, Martin Ovesný, Karel Fliegel, Miloš Klíma, and Guy M. Hagen
Opt. Express 22(24) 29805-29817 (2014)

Enhancing the isotropy of lateral resolution in coherent structured illumination microscopy

Joo Hyun Park, Jae Yong Lee, and Eun Seong Lee
Biomed. Opt. Express 5(6) 1895-1912 (2014)

Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy

Shwetadwip Chowdhury, Will J. Eldridge, Adam Wax, and Joseph A. Izatt
Biomed. Opt. Express 8(5) 2496-2518 (2017)