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Particle tracking by repetitive phase-shift interferometric super resolution microscopy

Abstract

Accurate and rapid particle tracking is essential for addressing many research problems in single molecule and cellular biophysics and colloidal soft condensed matter physics. We developed a novel three-dimensional interferometric fluorescent particle tracking approach that does not require any sample scanning. By periodically shifting the interferometer phase, the information stored in the interference pattern of the emitted light allows localizing particles positions with nanometer resolution. This tracking protocol was demonstrated by measuring a known trajectory of a fluorescent bead with sub-5 nm axial localization error at 5 Hz. The interferometric microscopy was used to track the RecA protein in Bacillus subtilis bacteria to demonstrate its compatibility with biological systems.

© 2018 Optical Society of America

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Supplementary Material (9)

NameDescription
Visualization 1       Demonstration of the accuracy of the system.
Visualization 1       Demonstration of the accuracy of the system.
Visualization 1       Demonstration of the accuracy of the system.
Visualization 2       100 nm fluorescent beads in 80% glycerol solution measured at 5 Hz.
Visualization 2       100 nm fluorescent beads in 80% glycerol solution measured at 5 Hz.
Visualization 2       100 nm fluorescent beads in 80% glycerol solution measured at 5 Hz.
Visualization 3       Tracking RecA-GFP protein fusion in Bacillus subtilis.
Visualization 3       Tracking RecA-GFP protein fusion in Bacillus subtilis.
Visualization 3       Tracking RecA-GFP protein fusion in Bacillus subtilis.

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Figures (6)

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