Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation

Abstract

We demonstrate stain-free, high-contrast, subcellular-resolution imaging of astroglial cells using epi-detected third-harmonic generation (THG). The astrocyte-imaging capability of THG is verified by colocalizing THG images with fluorescence images of astrocytes expressing a genetically encodable fluorescent reporter. We show that THG imaging with an optimized point-spread function can reliably detect significant subcellular features of astrocytes, including cell nuclei, as well as the soma shape and boundaries.

© 2019 Optical Society of America

Full Article  |  PDF Article
More Like This
Multimodal nonlinear-optical imaging of nucleoli

M. S. Pochechuev, A. A. Lanin, I. V. Kelmanson, A. S. Chebotarev, E. S. Fetisova, D. S. Bilan, E. K. Shevchenko, A. A. Ivanov, A. B. Fedotov, V. V. Belousov, and A. M. Zheltikov
Opt. Lett. 46(15) 3608-3611 (2021)

Cell-specific three-photon-fluorescence brain imaging: neurons, astrocytes, and gliovascular interfaces

A. A. Lanin, M. S. Pochechuev, A. S. Chebotarev, I. V. Kelmanson, D. S. Bilan, D. A. Kotova, V. S. Tarabykin, A. A. Ivanov, A. B. Fedotov, V. V. Belousov, and A. M. Zheltikov
Opt. Lett. 45(4) 836-839 (2020)

Label-free imaging of atherosclerotic plaques using third-harmonic generation microscopy

David M. Small, Jason S. Jones, Irwin I. Tendler, Paul E. Miller, Andre Ghetti, and Nozomi Nishimura
Biomed. Opt. Express 9(1) 214-229 (2018)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Supplementary Material (1)

NameDescription
Visualization 1       Stacks of TPEF and THG images taken by scanning the focus of the laser beam along the z-axis through layers V–VI in rat’s cortex.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved