Add to BibSonomy
Abstract
Optical resolution photoacoustic microscopy (OR-PAM) can map the cerebral vasculature at capillary-level resolution. However, the OR-PAM setup’s bulky imaging head makes awake mouse brain imaging challenging and inhibits its integration with other optical neuroimaging modalities. Moreover, the glass cranial windows used for optical microscopy are unsuitable for OR-PAM due to the acoustic impedance mismatch between the glass plate and the tissue. To overcome these challenges, we propose a lithium niobate based transparent ultrasound transducer (TUT) as a cranial window on a thinned mouse skull. The TUT cranial window simplifies the imaging head considerably due to its dual functionality as an optical window and ultrasound transducer. The window remains stable for six weeks, with no noticeable inflammation and minimal bone regrowth. The TUT window’s potential is demonstrated by imaging the awake mouse cerebral vasculature using OR-PAM, intrinsic optical signal imaging, and two-photon microscopy. The TUT cranial window can potentially also be used for ultrasound stimulation and simultaneous multimodal imaging of the awake mouse brain.
© 2022 Optica Publishing Group
Full Article | PDF ArticleMore Like This
Lisa Beckmann, Xian Zhang, Neil A. Nadkarni, Zhen Cai, Ayush Batra, David P. Sullivan, William A. Muller, Cheng Sun, Roman Kuranov, and Hao F. Zhang
Biomed. Opt. Express 10(10) 5235-5250 (2019)
Ajay Dangi, Sumit Agrawal, and Sri-Rajasekhar Kothapalli
Opt. Lett. 44(21) 5326-5329 (2019)
Shau Poh Chong, Conrad W. Merkle, Dylan F. Cooke, Tingwei Zhang, Harsha Radhakrishnan, Leah Krubitzer, and Vivek J. Srinivasan
Opt. Lett. 40(21) 4911-4914 (2015)