Abstract
Molecular order underpins many directed functions performed by cells, but its measurement in live cells and dynamic systems, across spatial and temporal scales, remains challenging. We have developed an optical microscope and computational methods that reveal the molecular orientation of single molecules and their ensembles in live cells—with a temporal resolution of 20 Hz, localization accuracy of 50 nm and orientation accuracy of less than 5 degrees. The microscope has enabled the study of some outstanding biological questions that hinge on how nanoscale molecules come together to form dynamic micron-scale assemblies in cells.
© 2016 Optical Society of America
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