Abstract
We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second).
© 2013 SPIE
PDF ArticleMore Like This
David Tyndall, Richard Walker, Krzysztof Nguyen, Rémi Galland, Jie Gao, Irène Wang, Meike Kloster, Antoine Delon, and Robert Henderson
80860S European Conference on Biomedical Optics (ECBO) 2011
Yongzhuang Zhou, Graham Hungerford, Guillem Carles, Vytautas Zickus, David McLoskey, Daniele Faccio, and Andrew R Harvey
CW1B.2 Computational Optical Sensing and Imaging (COSI) 2020
M. G. Tanner
1262720 European Conference on Biomedical Optics (ECBO) 2023