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Excited state fluorescence lifetimes of near-infrared fluorescent proteins iRFP682 and iRFP713 engineered from bacterial phytochromes were measured with a time-resolved flow cytometer. Average lifetimes ranged between 0.5 and 1.0 ns for n=5000 bacterial cells. We anticipate cell sorting for screening cells expressing mutated iRFP variants of high quantum yield.
© 2016 Optical Society of America
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