Fluorescence Lifetime Measurements in a Flow Cytometer by Amplitude Demodulation Using Digital Data Acquisition Technique

C. Deka; J.A. Stemkamp; Larry A. Sklar

doi:10.1364/LACA.1994.WD.11

Laser Applications to Chemical Analysis
Technical Digest Series (Optica Publishing Group, 1994),
paper WD.11
•https://doi.org/10.1364/LACA.1994.WD.11

Fluorescence Lifetime Measurements in a Flow Cytometer by Amplitude Demodulation Using Digital Data Acquisition Technique

C. Deka, J.A. Stemkamp, and Larry A. Sklar

Laser Applications to Chemical Analysis 1994

Wyoming United States
8–11 March 1994
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Poster Session 2 (WD)

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C. Deka, J. A. Stemkamp, and L. A. Sklar, "Fluorescence Lifetime Measurements in a Flow Cytometer by Amplitude Demodulation Using Digital Data Acquisition Technique," in Laser Applications to Chemical Analysis, Technical Digest Series (Optica Publishing Group, 1994), paper WD.11.

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Abstract

In this paper we demonstrate, for the first time, fluorescence lifetime measurements by amplitude demodulation from fluorescence signals with time dependent amplitudes in a flow cytometer using digital data acquisition techniques. The lifetime measurements in the flow cytometer have been compared to standard fluorescent lifetime measurements in bulk suspensions. The present technique provides a method for analyzing chemical and biological species based upon their individual excited state lifetime. We also discuss the factors influencing the accuracy of the measurements by this method and the possible solutions to those problems.

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