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  • Translational Biophotonics: Diagnostics and Therapeutics III
  • Technical Digest Series (Optica Publishing Group, 2023),
  • paper 126272H
  • https://doi.org/10.1117/12.2671025

Analysis of bacterial DNA by surface enhanced Raman spectroscopy

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Abstract

The detection of bacterial deoxyribonucleic acid (DNA) can be helpful to quickly identify a bacterium. A rapid SERS detection of bacterial DNA using microwave synthesized silver (Ag) nanoparticles (NPs) was reported. The microwave synthesizer technique offers quick synthesis of Ag NPs providing control pressure and uniform heat. The NPs based SERS can detect the genomic content of Escherichia coli (E. coli) NC_000913.3 and Pseudomonas aeruginosa (P. aeruginosa) NZ_CP012001 up to 50 ng/µL and 40 ng/µL respectively.

© 2023 SPIE

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