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Investigation of Collagen Fiber Organization in Cornea and Sclera using Quantitative SHG Microscopy

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Abstract

We propose the application of Fourier transform-second harmonic generation and polarization-resolved second-harmonic generation microscopy to quantify collagen fiber organization and content, respectively. As an example, the techniques are applied to the cornea and sclera.

© 2011 Optical Society of America

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