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Conferences > FiO > 2023 > JM4A > JM4A.68

Using Optical Microscopy to Study Synaptic Vesicle Docking and Rapid Calcium-Mediated Fusion with an In Vitro Reconstitution of the SNARE Machinery

Atrouli Chatterjee and James E. Rothman

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Abstract

To study the molecular interactions involved in vesicle docking and subsequently in Ca2+-triggered release, we introduce a reconstitution of the genetically-validated core protein machinery within a geometry the enables the detailed characterization of vesicle fate.

© 2023 The Author(s)

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