Abstract
Detection in High Performance Liquid Chromatography (HPLC) is most often done by optical methods1. The most popular detection modes, in order of importance are, absorption, fluorescence, refractive index (RID) or optical activity (OAD). It is known2 that the measured changes in those physical properties are always associated with changes in refractive index (Δn) in the mobile phase. This coupling of refractive index (RI) usually shows up in the response of detectors as non-linearity2, increased noise level or, even spurious response, which all have detrimental effects on the quantitative evaluation of chromatograms.
© 1992 Optical Society of America
PDF ArticleMore Like This
J. Vaitkus, M. Petrauskas, A. Banaitis, and J. Kolenda
PTh033 International Quantum Electronics Conference (IQEC) 1992
Chang Su, M. O. Scully, M. Fleischhauser, C. H. Keitel, and Shi-Yao Zhu
MoO1 International Quantum Electronics Conference (IQEC) 1992
M. A. Taubenblatt and J. S. Batchelder
TuG4 OSA Annual Meeting (FIO) 1992